Code No. Product Size 318-03971 T4 GT7 DNA 70µg 314-03973 70µg×5
Storage temperature: -20℃
Storage Condition: 10mM Tris-HCl (pH7.9), 1mM EDTA
Source: Bacteriophage T4 GT7〔amC87 (42-), amE51 (56-), NB5060 (△rIIB-denB-ac), unf39 (alc)〕〔E.coli MC1061 (Su-)〕
Molecular Weight: 1.08×10^8 daltons (166 kbp)
Concentration: 0.2-0.5 μg/μl (Varies depending on lot)
Purity: A260/A280≈1.8 (Varies depending on lot)
※As cytosine is not hydroxy-methylated, T4 GT7 DNA is suitable for restriction enzyme reaction.
Reference
1) Wilson, G. G., Young, k. k. Y., Edlin, G. J. and Konigsberg, W. : Nature, 280, 80 (1979)
2) Yonesaki, T., Ryo, Y., Minagawa, T. and Takahashi, H.: Eur.J. Biochem., 148, 127 (1985)
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