DGGE Marker

Product	Package unit	Code No.	Concentration
DGGE Marker �T (5 fragments)	30µl	318-06411	50 ng/5µl
	150µl	314-06413
	150µl X 5	312-06414
DGGE Marker �U (10 fragments)	30µl	311-06401	100 ng/5µl
	150µl	317-06403
	150µl X 5	315-06404
DGGE Marker �V (10 fragments)	30µl	315-06921	100 ng/5µl
	150µl	311-06923
	150µl X 5	319-06924
DGGE Marker �W (8 fragments)	30µl	312-06931	80 ng/5µl
	150µl	318-06933
	150µl X 5	316-06934
DGGE Marker �X (9 fragments)	30µl	319-06941	90 ng/5µl
	150µl	315-06943
	150µl X 5	313-06944

 

DGGE Marker is designed for DNA standards of DGGE (denaturing gradient gel electrophoresis).

*For research use only. Not for use in diagnostic or therapeutic purpose.

DGGE Marker contains Loading Buffer. Apply directly to the polyacrylamide gel. SYBR® Gold, SYBR® Green I or EtBr can be used for staining of acrylamide gel.

Form : 10mM Tris-HCl(pH8.0)，10mM EDTA(pH8.0)，0.025% Bromophenol Blue，35% Glycerol

DGGE Marker �V, �W and �X were developed by National Institute for Agro-Environmental Sciences (NIAES) for the analysis of flora/fauna of bacteria, fungi and nematode in the soil. NIAES has conducted a project of "Development of soil biodiversity analysis system with environmental DNA", which was supported by the Ministry of Agriculture, Forestry, and Fisheries, Japan. For more details on the DGGE analysis, please refer to the journal of the Japanese Society of Soil Microbiology, "Soil and Microorganism", Vol.62, No.1 (April 2008).

Store at -20 ℃

DGGE was performed with a DCode System (Bio-Rad).5μl of DGGE Marker was applied to polyacrylamide gel. After electrophoresis, the fragments of DGGE Marker were stained with SYBR® Gold.

*Electrophoresis patterns of DGGE Markers are different by DGGE condition (voltage, time,temperature, concentration of denaturant and acrylamide).

　DGGE Marker Leaflet

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>Japanese (DGGE Marker I, II)

>Japanese (DGGE Marker III, �W,�X)