ISOFECAL for Beads Beating is a kit for extracting DNA from the fecal samples.

ISOFECAL for Beads Beating uses both chemical lysis by a surface-active agent and physical disruption of cells by beads beating as the DNA extraction method. As a result, DNA can be extracted from microorganisms having strong cell walls, and fecal DNA reflecting actual fecal microflora can be obtained.

However, users should be aware that DNA extracted by ISOFECAL for Beads Beating may have been subjected to physical shearing from beads beating.

All the reagents included in ISOFECAL for Beads Beating can be stored at room temperature.

However, for the Precipitation Solution, the Wash Solution and the Ethachinmate, we recommend that care be taken to prevent contamination at the time of use (contamination by fungi and bacteria), with storage at a low temperature (2-10°C) after opening.

• This product is a reagent for research and cannot be used for medical or other objectives.
• This product should be handled only by persons having basic knowledge of reagents.
• Handle this product in accordance with the descriptions in the manual.
• We are not responsible for problems caused if this product is not handled in accordance with the manual.
• A patent has been filed for fecal DNA extraction with ISOFECAL for Beads Beating by the University of Tokyo TLO . Nippon Gene has been licensed to practice the fecal DNA extraction method by the University of Tokyo TLO .

　ISOFECAL for Beads Beating Manual (First edition)

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