ISOIL is a kit for extracting DNA from soil samples.

By using an extracting solution with a special composition, DNA can be extracted not only from non-volcanic ash soil but also from volcanic ash soil, something which has been considered difficult to achieve up to now.

With ISOIL, DNA is isolated with a heat extraction method in the presence of a surface-active agent. Since soil DNA is extracted without applying physical force, high molecular weight DNA can be obtained. Therefore, soil DNA extracted with ISOIL is suitable for applications involving gene resources, e.g., the construction of a metagenome library.

However, please note that microorganisms having strong cell walls may not be destroyed.

Please use ISOIL for Beads Beating, which uses disruption by beads beating to perform group structure analysis of soil microorganisms through PCR-DGGE analysis and estimation of soil biomass by quantitative determination of soil DNA.

All the reagents included in ISOIL can be stored at room temperature.
However, for the Precipitation Solution, the Wash Solution and the Ethachinmate, we recommend that care be taken to prevent contamination at the time of use (contamination by fungi and bacteria), with storage at a low temperature (2-10°C) after opening.

• This product is a reagent for research and cannot be used for medical or other objectives.
• This product should be handled only by persons having a basic knowledge of reagents.
• Handle this product in accordance with the descriptions in the manual.
• We are not responsible for problems caused if this product is not handled in accordance with the manual.
• A patent has been filed for soil DNA extraction with ISOIL by the University of Tokyo TLO. Nippon Gene has licensed the soil DNA extraction method from the University of Tokyo TLO.

　ISOIL (Second edition)

>ISOIL for Beads Beating

>Japanese