Ligation-Convenience Kit

Product	Size	Code No.
Ligation-Convenience Kit	100 ligations	319-05961
	10 ligations	315-05963

The first important step of gene cloning is to ligate the target gene to a vector DNA. The efficiency of the ligation reaction varies depending on the shape of the DNA terminal, and until now the ligation reaction has needed to be optimized for each reaction. Further, the ligation reaction requires 1-16 hours using a conventional T4 DNA ligase, and it has been difficult to obtain high transformation efficiency. The Ligation-Convenience Kit can solve these problems and provide highly efficient ligation in a short period of time.

• This product is a kit for performing DNA ligation rapidly and simply.
• The 2×Ligation Mix contains all the components required for DNA ligation such as the reaction buffer, ATP, DTT, T4 DNA Ligase, etc. The DNA ligation reaction can be performed by adding an equal volume of 2×Ligation Mix to the DNA solution.
• By using this kit, the ligation reaction can be performed in 5-30 min regardless of the shape of the DNA terminal. Further, the DNA solution in which the ligation reaction has been completed can be used for transformation and in vitro packaging as-is.

Reagent	For 100 ligations*	For 10 ligations*
2×Ligation Mix	250µl×4tubes	100µl×1tubes

 

* When used in 20 μl reaction system

Storage at −20℃

・ At the time of use, thaw completely on ice and mix well by pipetting.
・ No reduction in reaction efficiency was found in up to 50 cycles of freezing and thawing.

Ligation-Convenience Kit manual

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>Japanese