Agarose S Tablet
Agarose for Gel Electrophoresis| Product Name | Code No. | Size | Price | Note |
|---|---|---|---|---|
Agarose S Tablet |
312-06073 |
0.5 g x 10 tablets (5 g) |
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Agarose S Tablet |
316-06071 |
0.5 g x 140 tablets (70 g) |
Product Description
Agarose S Tablet
Agarose S Tablet is provided as a tablet format, one tablet is 0.5g of Agarose S. The innovative product enables preparation of agarose gels without weighing.
Features
・0.5g of Agarose S / one tablet・low EEO・DNase free, RNase free・Recommended Gel Concentration: 0.8 - 2 %・Nucleic Acid Fragment Separation Range: 0.5 - 30 kbp
Applications
- Gel Electrophoresis for separation of DNA fragments
- Pulsed-Field Electrophoresis (1 - 2,000 kbp)
- Recovered DNA*1
- Blotting
*1 Recovered DNA from preparative Agarose gels is checked that it can be used for downstream applications (cleavage by restriction endonucleases, ligation by T4 DNA ligase, E. coli transformation by ligated DNA.)
| Gel strength | ≥ 1,200 g/cm2 (1.5%) |
|---|---|
| Melting point | ≤ 90°C (1.5%) |
| Gelling temperature | 37 - 39°C (1.5%) |
| Sulfate content (SO4) | ≤ 0.1% |
| Water content | ≤ 10% |
| Electroendosmosis (-Mr) | ≤ 0.1 (Low EEO) |
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Usage
Protocol
*1 Stand until the tablet disintegrates.
Preparation of 1% Agarose S gel
- Add one tablet to 50 ml of TAE or TBE buffer, leave for 5 minutes at room temperature.
- Leave for 5 minutes at room temperature to allow the tablet to fall apart. *1
- Shake gently and heat in a microwave oven or the like after confirming there is no solid material.
Usage Note
Do not heat the solution before the tablets have fallen apart.
When heated in the presence of solid materials with a microwave oven, a gel film is formed on the surface of the solid materials preventing further melting of Agarose, as shown in the bad example below.
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Quick Reference Chart of Agarose Tablet
concentration
Separation of DNA markers in Agarose S
Agarose S 1% Gel
| Lane | 1 |
2 |
3 |
4 |
| λ Marker | Marker 1 | Marker 2 | Marker 3 | Marker 6 |
| DNA fragments (kbp) |
23.13 9.42 6.56 4.36 2.32 2.02 0.56 0.13 |
21.23 5.15 4.98 4.27 3.52 2.02 1.91 1.58 1.38 0.95 0.83 0.56 0.13 |
23.13 21.23 9.42 7.42 6.56 5.81 5.65 4.88 4.36 3.52 2.32 2.02 0.56 0.13 |
19.33 7.74 6.22 4.26 3.47 2.69 1.88 1.49 0.93 0.42 0.07 |
Effect of Agarose concentration on the migration distance of bands
Molecular weight markers were electrophoresed using Agarose S 0.5% gel, 1% gel and 2% gel, and the effect of the Agarose gel concentration on the migration distance of the band was investigated. (Use as a reference for determining the concentration when a gel is prepared.)
Ladder Markers
Lane 1：OneSTEP Ladder 1 kb
Lane 2：OneSTEP Ladder 500
Lane 3：OneSTEP Ladder 100
Lane 4：Smart Ladder
Lane 5：OneSTEP Marker 1 (λ/Hind III digest)
Lane 6：OneSTEP Marker 6 (λ/Sty I digest)
Lane 7：OneSTEP Marker 4 (φX174/Hae III digest)
Lane 8：OneSTEP Marker 5 (φX174/Hinc II digest )
0.5% Agarose S |
1% Agarose S |
2% Agarose S |
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 |
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| Lane | 1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
| DNA fragments (kbp) |
10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 |
5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 |
2.0 1.8 1.6 1.4 1.2 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 |
10.0 8.0 6.0 5.0 4.0 3.0 2.5 2.0 1.5 1.0 0.8 0.6 0.4 0.2 |
23.1 9.4 6.6 4.4 2.3 2.0 0.6 0.1 |
19.3 7.7 6.2 4.3 3.5 2.7 1.9 1.5 0.9 0.4 0.07 |
1.35 1.08 0.87 0.60 0.31 0.28 0.27 0.23 0.19 0.12 0.07 |
1.057 0.770 0.612 0.495 0.392 0.345 0.341 0.335 0.297 0.291 0.210 0.162 0.079 |
Recommended Gel Concentration
- 1 % Agarose S gel (greater than or equal to 1 kb DNA)
- 2 % Agarose S gel (less than 1 kb DNA)
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Data Sheets
SDS(Safety Data Sheet)
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Related Information
Note
- This product is for research use only.
Related Products
- Agarose S (Gel range: 0.5 - 2%)
- Agarose XP (Gel range: 1 - 4%)
- Agarose H (Gel range: 0.2 - 1%)
Contact us
- Distributor
- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
- E-mail：Web Contact Form
