RNA Ladder (0.125 - 6.0 kb)
RNA size marker| Product Name | Code No. | Size | Price | Note |
|---|---|---|---|---|
| RNA Ladder (0.125-6.0 kb) | 311-06261 | 25 µl |
||
| RNA Ladder (0.125-6.0 kb) | 317-06263 | 25 µl x 2 |
Product Description
This product is an RNA size standard ladder marker consisting of 9 bands prepared by in vitro transcription.
RNA Ladder (0.125-6.0 kb)
| Fragment | base |
|
| 1 | 6,000 | |
| 2 | 4,000 | |
| 3 | 3,000 | |
| 4 | 2,000 | |
| 5 | 1,500 | |
| 6 | 1,000 | |
| 7 | 500 | |
| 8 | 250 | |
| 9 | 125 |
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Product Components
| Component | Quantity | Storage | Description |
|---|---|---|---|
| RNA Ladder | 25 μl x 1 | -80°C | RNA concentration: about 1 μg/μl Storage conditions: 10 mmol/l Tris-HCl(pH 8.0), 1 mmol/l EDTA(pH 8.0) |
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Usage
| RNA Ladder | 1 μl | RNA sample (3 μl) |
|---|---|---|
| TE (pH 8.0) | 2.5 μl | |
| Formamide | 5 μl | RNA sample buffer (9 μl) |
| 10 x MOPS (pH 7.0)*1 | 1.5 μl | |
| Formaldehyde | 2 μl | |
| 1 mg/ml Ethidium Bromide | 0.5 μl |
Heat at 65°C for 5min. After heat treatment, cool rapidly.
Mix with 2.5 μl of RNA loading buffer*2 and electrophorese in formaldehyde denaturing agarose gel.
- *1 10 x MOPS (pH 7.0): 200mM MOPS, 50mM NaOAc, 10mM EDTA, pH adjustment with NaOH
- *2 RNA loading buffer: 50% Glycerol, 1 mmol/l EDTA(pH 8.0), 0.01% Bromophenol blue
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Data Sheets
SDS(Safety Data Sheet)
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Related Information
Purity
- 9 bands are confirmed after electrophoresing 1-2 μl of the product in 1% denaturing agarose gel and staining with ethidium bromide.
Note
- Since this product is easily degraded by RNA degrading enzymes, handle with care.
- This product is for research use only.
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Contact us
- Distributor
- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
- E-mail：Web Contact Form
