RNA Ladder (0.125 - 6.0 kb)

RNA size marker
Top> Reagents for research> RNA Ladder (0.125-6.0 kb)
Product Name Code No. Size Price Note
RNA Ladder (0.125-6.0 kb) 311-06261
25 µl
   
RNA Ladder (0.125-6.0 kb) 317-06263
25 µl x 2
   

Product Description

This product is an RNA size standard ladder marker consisting of 9 bands prepared by in vitro transcription.

RNA Ladder (0.125-6.0 kb)

Fragment base

onestep-marker1
1% Agarose S
(formaldehyde denaturing)
EtBr staining

1 6,000
2 4,000
3 3,000
4 2,000
5 1,500
6 1,000
7 500
8 250
9 125

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Product Components

RNA Ladder (25 µl)
Component Quantity Storage Description
RNA Ladder 25 μl x 1 -80°C RNA concentration: about 1 μg/μl
Storage conditions: 10 mmol/l Tris-HCl(pH 8.0), 1 mmol/l EDTA(pH 8.0)

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Usage

Usage
RNA Ladder 1 μl RNA sample (3 μl)
TE (pH 8.0) 2.5 μl
Formamide 5 μl RNA sample buffer (9 μl)
10 x MOPS (pH 7.0)*1 1.5 μl
Formaldehyde 2 μl
1 mg/ml Ethidium Bromide 0.5 μl

Heat at 65°C for 5min. After heat treatment, cool rapidly.

Mix with 2.5 μl of RNA loading buffer*2 and electrophorese in formaldehyde denaturing agarose gel.

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Data Sheets

SDS(Safety Data Sheet)

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Related Information

Purity

Note

Related Products

Contact us

Distributor
FUJIFILM Wako Pure Chemical Corporation
Produced by
NIPPON GENE CO., LTD.

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