Agarose

Agarose from Nippon Gene is specially purified for separating and recovering DNA fragments, and 9 types are available for different usages.
Since the strict quality control is performed on the following items for every agarose preparation, the sufficient separation and the purity of recovered DNA are guaranteed.

• Gel strength (by the method of The Agar-Agar Products Association of Japan)
• Melting point
• Gelling temperature
• Sulfate content (SO₄)
• Water content
• Electroendosmosis (-Mr)
• Check for recovered DNA
  1) Cleavage by restriction endonucleases
  2) Ligation by T4 DNA ligase
  3) E. coli transformation by ligated DNA (pBR322 and the like)

*This product is sold as a reagent (for research) and is not for pharmaceutical use.

Use	S	S<Tablet>	HS	L	H	21	X	XP	GB
Concentration range	0.5-2%		0.5-2%	0.5-2%	0.2-1%	2-5%	2-6%	1-4%	-
Separation range (kbp)	0.5-30		0.5-30	0.5-30	1-200	0.01-1.0	0.01-1.0	0.01-20	-
Electrophoresis of nucleic acid (10-1,000bp)						◯	◯	◯
Electrophoresis of nucleic acid (<30kbp)	◯	◯	◯	◯	◯			△(<20kbp)
Electrophoresis of nucleic acid (>30kbp)					◯
Pulse field electrophoresis (1-2,000kbp)	◯	◯	◯	◯				◯
Pulse field electrophoresis (>2,000kbp)					◯
DNA sample preparation for pulse field electrophoresis				◯					◯
Recovery of nucleic acid	◯	◯	◯	◯	◯	◯	◯	◯
Blotting of nucleic acid	◯	◯	◯	◯	◯	◯	◯	◯
No need for weighing agarose		◯				◯ (Stick type)

Product	Package unit	Code No.
Agarose S	5 g	316-01191
	100 g	312-01193

Agarose S is prepared for electrophoresis of nucleic acid fragments.

■Specification

Gel strength	≧1,200g/cm2(1.5%)
Melting point	90℃(1.5%)
Gelling temperature	37-39℃(1.5%)
Sulfate content (SO4)	≦0.1%
Water content	≦10%
Electroendosmosis (-Mr)	≦0.1

■Example of electrophoresis

Lane 1. Marker 1 (λ/Hind III digest) Lane 2. Marker 2 (λ/Hind III・EcoR I double digest) Lane 3. Marker 3 (λ/Hind III＋λ/EcoR I digest mixture) Lane 4. Marker 6 (λ/StyI digest) 1% Agarose S

> Reference materials： Effect of Agarose concentration on the migration distance of bands

Product	Package unit	Code No.
Agarose S Tablet	0.5 g×10	312-06073
	0.5 g×140	316-06071

Agarose S Tablet is a tablet product containing 0.5g of Agarose S/tablet, and an innovative product making the weighing of Agarose unnecessary when the Agarose gel is prepared. This is used for electrophoresis of fragments of nucleic acid.

■Specification

Gel strength	≧1,200g/cm2(1.5%)
Melting point	≦90℃ (1.5%)
Gelling temperature	37-39℃ (1.5%)
Sulfate content (SO4)	≦0.1%
Water content	≦10%
Electroendosmosis (-Mr)	≦0.1

> For use

Product	Package unit	Code No.
Agarose HS	2 g	318-01433
	100 g	312-01431

Agarose HS is a product in which the precision of Agarose S type is enhanced and the gel strength is increased.
It is suitable for Southern blotting and the like.

■Specification

Gel strength	≧1,600g/cm2(1.5%)
Melting point	≦93℃(1.5%)
Gelling temperature	37-39℃(1.5%)
Sulfate content (SO4)	≦0.1%
Water content	≦10%
Electroendosmosis (-Mr)	≦0.1

■Example of electrophoresis

Example of a genetic diagnosis using mouse tail DNA Sample: tail DNA of a transgenic mouse*) 　 *) The human transthyretin gene, which is the causative gene of familial amyloid polyneuropathy,was introduced. 1: The introduced human transthyretin gene 2, 3: The junction part between the introduced human transthyretin gene and mouse chromosomal DNA 4: The mouse MHC EKα gene (single copy) used as a control 　0.8% Agarose HS 　Materials provided by Research Center for Genetic Information , Kyushu University

Product	Package unit	Code No.
Agarose L	2 g	319-01181
	25 g	317-01182

Agarose L is a low melting type agarose , which melts again at around 65℃ and is suitable for separation and purification of nucleic acid fragments.

■Specification

Gel strength	≧450g/cm2(1.5%)
Melting point	≦65℃(1.5%)
Gelling temperature	≦30℃(1.5%)
Sulfate content (SO4)	≦0.1%
Water content	≦10%
Electroendosmosis (-Mr)	≦0.1

Product	Package unit	Code No.
Agarose H	1 g	315-01203
	10 g	319-01201
	25 g	317-01202

Agarose H is a high strength type agarose , which maintains a high strength at a low agarose concentration and is suitable for electrophoresis of large nucleic acid fragments.

■Specification

Gel strength	≧2,600g/cm2(1.5%)
Melting point	boil(1.5%)
Gelling temperature	37-39℃(1.5%)
Sulfate content (SO4)	≦0.2%
Water content	≦10%
Electroendosmosis (-Mr)	≦0.2

■Example of electrophoresis

・Lane 1. Marker 7GT (T4GT7+T4 GT7/Bgl I digest mixture), 0.4µg ・Lane 2. Marker 8GT (T4GT7＋T4 GT7/Bgl II digest mixture), 0.4µg Run at 20V for 15hr. 0.3% Agarose Ｈ

Product	Package unit	Code No.
Agarose 21	25 g	313-03242
	100 g	319-03244
	(Stick type) 3 g	311-03243
	(Stick type) 3 g×25	315-03241

Agarose for low molecular weight nucleic acid separation

Agarose 21 is developed for separating nucleic acid of 1,000bp or less and is suitable for electrophoresing short chain DNA such as PCR products and restriction endonuclease fragments, and RNA.

This product is provided as the stick type and the bottle type, and in the case of the stick type, there is no need of weighing agarose powder, and 100ml of 3% agarose gel can be prepared from one stick (3g). Further, the handling of the gel during the manipulation is easy because this product has a superior solubility and high gel strength.

■Specification

Gel strength	≧800g/cm2(3%)
Melting point	≦85℃(3%)
Gelling temperature	34-38℃(3%)
Sulfate content (SO4)	≦0.1%
Water content	≦10%
Electroendosmosis (-Mr)	≦0.1

■User guide

• Agarose concentration: 3% (normal)
• Gel thickness: 3mm (Clear electrophoresis patterns can be obtained by limiting the gel thickness to about 3mm)
• Buffer: TAE buffer or TBE buffer

■Precautions

At a gel concentration higher than 5%, the solution becomes highly viscous and may be difficult to handle. When melting, cover the container with a plastic wrap to prevent buffer evaporation and open a pinhole.

■Data

Comparison with Agarose X	Test for the minimum detectable amount of DNA	Electrophoresis in Mini-gel （ Mupid®)
・M4 : Marker 4 (φX174/Hae III digest) ・M5 : Marker 5 (φX174/Hinc II digest) ・M9 : Marker 9 (φX174/Hinf I digest) ・M10 : Marker 10 (pBR322/Msp I digest) Each 0.5µg run at 60V for 4hr. in TAE Buffer 3% Agarose 21, 3% Agarose X 12.5cm×10cm×3mm	Marker 4 (φX174/ Hae III digest) run at 60V for 4hr. in TAE Buffer 3% Agarose 21 12.5cm×15cm×3mm	・M4 : Marker 4 (φX174/Hae III digest) ・M5 : Marker 5 (φX174/Hinc II digest) ・M9 : Marker 9 (φX174/Hinf I digest) ・M10 : Marker 10 (pBR322/Msp I digest) 1 : 0.1µg 2 : 0.5µg run at 50V for 80min. in TAE Buffer 3% Agarose 21

Product	Package unit	Code No.
Agarose X	3 g	319-02683
	25 g	311-02682
	100 g	313-02681

Agarose for low molecular weight nucleic acid separation

Agarose X is developed for separating nucleic acid of 1,000bp or less and is suitable for electrophoresing short chain DNA such as PCR products and restriction endonuclease fragments, and RNA. Further, the handling of the gel during the manipulation is easy because this product has a superior solubility and high gel strength.

■Specification

Gel strength	≧1,200g/cm2(4%)
Melting point	≦92℃(4%)
Gelling temperature	31-39℃(4%)
Sulfate content (SO4)	≦0.15%
Water content	≦10%
Electroendosmosis (-Mr)	0.06-0.14

■User guide

• Agarose concentration: 3% (normal)
• Gel thickness: 3mm (Clear electrophoresis patterns can be obtained by limiting the gel thickness to about 3mm)
• Buffer: TAE buffer or TBE buffer

■Precautions

At a gel concentration higher than 6%, the solution becomes highly viscous and may be difficult to handle. When melting, cover the container with a plastic wrap to prevent buffer evaporation and open a pinhole. The solution may be slightly yellowish but there is no problem of the quality.

Product	Package unit	Code No.
Agarose XP	3 g	314-06511
	25 g	312-06512
	100 g	310-06513
	500 g	316-06515

• A low melting type agarose .
• Suitable for electrophoresing 10bp-1,000bp (short chain fragments) (2.5% gel).
• Fragments ≦20kbp (long chain fragments) can be electrophoresed (1% gel).
• Very economical because fragments of almost all the size (10bp-20kbp ) can be electrophoresed .

■Specification

Gel strength	≧700g/cm2(3%)
Melting point	≦70℃(3%)
Gelling temperature	≦35℃(3%)
Sulfate content (SO4)	≦0.1%
Water content	≦10%
Electroendosmosis (-Mr)	≦0.1

Product	Package unit	Code No.
Agarose GB	1 g	310-01513
	10 g	314-01511

Agarose for preparing DNA for pulse field electrophoresis

Agarose GB is a product for preparing and maintaining the chromosomal DNA in an intact form used for pulse field electrophoresis. The restriction endonuclease reaction can be directly performed in this agarose gel piece.

■Specification

Melting point	≦65℃(1.5%)
Gelling temperature	≦30℃(1.5%)
Sulfate content (SO4)	≦0.1%
DNase	free

■Example of electrophoresis

Pulse field electrophoresis pattern of Saccharomyces cerevisiae chromosomal DNA (245-2,500kbp) prepared using Agarose GB. (1% Agarose S)

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